But if it is a plant sample, it is mainly represented by actin and GAPDH. Sample Source: in mammalian tissue or cell, it is mainly represented by Actin, Tubulin, GAPDH, Lamin B1, PCNA. When choosing a right loading control antibody as an internal reference, you need to pay attention to the following aspects. They can also be used to compare the expression of target protein in different cell tissues under different conditions. What are the important factor when you choose the suitable loading control antibodies in experiment?Īs mentioned earlier, loading control antibodies are reference objects that can monitor whether the whole test system is normal, such as protein extraction process, membrane transfer system, color development system, etc. Additionally, in the last section, we introduce five common loading control proteins and antibodies.Ģ. In this article, we primarily focus on loading control antibodies and list several questions about CUSABIO loading control antibodies. HRP- and biotin-conjugated versions of these antibodies are also available. Housekeeping proteins, such as GAPDH, tubulin, and actin, represent some of the well-known loading control proteins.ĬUSABIO offers a wide selection of monoclonal loading control antibodies for GAPDH, α-tubulin, and β-actin for several species. Loading control proteins, provide a reference to evaluate the changes in expression levels of target proteins and help to ensure that any increase or decrease in target protein levels is due to your experimental manipulations, and not due to errors in the Western blot procedure. Loading control antibodies are known to be constitutively and stably expressed at high levels in almost all tissues and cells. Isotype control antibodies are used as negative controls to help distinguish non-specific background signal from specific antibody signal. Isotype control antibodies refer to a group of primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application, especially in flow cytometry experiments. Generally speaking, control antibody can be divided into two groups, including isotype control antibodies and loading control antibodies.
#Good loading control western blot series
Control antibodies, as the name means, are a series of internal control antibodies.
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